CHRM2/CHO
CBP71427
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产品数据库
| I. Background | |
| 毒蕈碱乙酰胆碱受体（MAChRs，简称 M 受体）参与中枢神经系统的运动控制、体温 调节、心血管调节和记忆，介导周围神经系统的平滑肌收缩、腺体分泌和心率、调节心肌 收缩力。CHRM2 属于 GPCR，是 MAChRs 的 5 种亚型之一，可以与 Gi/o 偶联。CHRM2 是心脏和脑干中最主要的毒蕈碱受体亚型。CHRM2 顺利获得 Gi/o 信号精密抑制心脏与神经元 过度活动，维持内环境稳态。其功能失衡直接导致心动过缓、癫痫易感性升高及抗抑郁治 疗抵抗，是神经心血管药物设计的核心靶标。 | |
| II. Description | |
HRM2 CHO 药靶模型很好的模拟了体内 CHRM2 的信号转导过程，原理见下图所示。 Figure 1. CHRM2 CHO 细胞模型原理图 |
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| III. Introduction | |
| Host Cell: |
CHO |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | F12K+10%FBS+5μg/ml puromycin |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen immediately upon delivery |
| Application(s): |
Functional assays for CHRM2 |
| Transducer: | Gi |
| IV. Description of Host Cell Line | |
| Organism: | Hamster |
| Tissue: | Ovary |
| Morphology: | Epithelial |
| Growth Properties: | Adherent |
| V. Representative Data | |
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Figure 2. Recombinant CHRM2/CHO constitutively expressing CHRM2. |
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Figure 3. HTRF cAMP Assay With CHRM2 CHO Cells (C3). |
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